SERPINB13 ELISA Kit (Human) (OKEH02431)

Catalog No: OKEH02431

Size:96T

Human SERPINB13 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology SERPINB13 ELISA Kit (Human) (OKEH02431) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human SERPINB13 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SERPINB13 and incubated. The SERPINB13 found in the sample or standards competes with the biotinylated SERPINB13 for limited binding sites on the immobilized anti-Human SERPINB13 antibody. Excess unbound biotinylated SERPINB13 and sample or standard SERPINB13 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SERPINB13 captured in the well and inversely proportional to the amount of SERPINB13 which was contained in the sample or standard.
• ELISA Kit Range: 1.56-100ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 8.4% (n = 20)
  Mean Inter-assay CV%: < 11.1% (n = 20)
• ELISA Kit Component:
  • Anti-SERPINB13 Microplate: 96 Wells (12 x 8 Well strips)
  • SERPINB13 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X SERPINB13 Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.23 ng/mL
• Specificity: Natural and recombinant Human Serpin B13
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: SERPINB13
• Gene Full Name: serpin family B member 13
• Alias Symbols: haCaT UV-repressible serpin;headpin;HSHUR7SEQ;HUR7;HURPIN;peptidase inhibitor 13;PI13;PI-13;protease inhibitor 13 (hurpin, headpin);proteinase inhibitor 13;serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 13;serpin B13;serpin peptidase inhibitor, clade B (ovalbumin), member 13;UV-B repressed sequence, HUR 7.
• NCBI Gene Id: 5275
• Protein Name: Q9UIV8
• Description of Target: Serpin B13
• Uniprot ID: https://www.uniprot.org/uniprot/Q9UIV8
• Protein Accession #: NP_001294852.1
• Nucleotide Accession #: NM_001307923.1
• Protein Size (# AA): 391
• Molecular Weight: 44 kDa