Catalog No: OKCD07227
Size:96WELLS
Price: $650.00
SKU
OKCD07227
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Datasheets/ManualsPrintable datasheet for SERPINA6 ELISA Kit (Mouse) (OKCD07227)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corticosteroid Binding Globulin (CBG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Corticosteroid Binding Globulin (CBG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corticosteroid Binding Globulin (CBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Corticosteroid Binding Globulin (CBG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticosteroid Binding Globulin (CBG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticosteroid Binding Globulin (CBG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
SERPINA6 Microplate96 Wells (12 x 8 Well strips)
SERPINA6 Lyophilized Standard2
100X SERPINA6 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 0.34ng/mL
Gene SymbolSerpina6
Gene Full Nameserine (or cysteine) peptidase inhibitor, clade A, member 6
Alias SymbolsCbg;corticosteroid-binding globulin;serine (or cysteine) proteinase inhibitor, clade A, member 6;serpin A6;transcortin.
NCBI Gene Id12401
Protein NameCorticosteroid-binding globulin
Description of TargetMajor transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.
Uniprot IDQ06770
Protein Accession #NP_001747.2
Nucleotide Accession #NM_001756.3
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