Catalog No: OKEH00287
Size:96T
Price: $525.00
SKU
OKEH00287
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SERPINA6 ELISA Kit (Human) : 96 Wells (OKEH00287)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SERPINA6 ELISA Kit (Human) (OKEH00287) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINA6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for SERPINA6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINA6 captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)
ELISA Kit Component
ComponentAmount
SERPINA6 Microplate96 Wells (12 x 8 Well strips)
SERPINA6 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SERPINA6 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.73 ng/mL
SpecificityNatural and recombinant Human Corticosteroid-binding globulin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSERPINA6
Gene Full Nameserpin family A member 6
Alias SymbolsCBG;corticosteroid-binding globulin;serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6;serpin A6;serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6;transcortin.
NCBI Gene Id866
Protein NameP08185
Description of TargetCorticosteroid-binding globulin
Uniprot IDhttps://www.uniprot.org/uniprot/P08185
Protein Accession #NP_001747.2
Nucleotide Accession #NM_001756.3
Protein Size (# AA)405
Molecular Weight45 kDa
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