Catalog No: OKCA00756
Size:96 Wells
Price: $754.00
SKU
OKCA00756
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
1:2009792-102
1:4009182-96
1:80010398-109
1:16008782-96
ELISA Kit PrincipleAviva Systems Biology SERPINA5 ELISA Kit (Human) (OKCA00756) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINA5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPINA5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SERPINA5 captured in the well.
ELISA Kit Range0.156 ng/mL-10 ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9085-96
EDTA Plasma (n=4)8784-92
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level SERPINA5 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level SERPINA5 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
SERPINA5 Microplate96 Wells (12 x 8 Well strips)
SERPINA5 Lyophilized Standard2
100X Biotinylated SERPINA5 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernatant And Urine
Sensitivity0.039 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSERPINA5
Gene Full NamePlasma serine protease inhibitor
Alias SymbolsPCI, PAI3, PAI-3, PCI-B, PROCI, PLANH3
NCBI Gene Id5104
Protein NamePlasma serine protease inhibitor
Description of TargetHeparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and proinflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary-type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue-and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as a hydrophobic hormone carrier by its binding to retinoic acid.
Uniprot IDP05154
Protein Accession #NP_788819.1
Nucleotide Accession #NM_176646.3
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