SERPINA5 Chemi-Luminescent ELISA Kit (Human) (OKCD03858)

Catalog No: OKCD03858

Size:96WELLS

Human SERPINA5 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  sodium citrate plasma(n=5)	91-104%	81-101%	78-99%	78-98%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Protein C Inhibitor (PCI). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Protein C Inhibitor (PCI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Protein C Inhibitor (PCI) level in the sample or standard.
• ELISA Kit Range: 109.7-80,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  sodium citrate plasma(n=5)	87-96	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein C Inhibitor (PCI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein C Inhibitor (PCI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SERPINA5 Microplate: 96 Wells (12 x 8 Well strips)
  • SERPINA5 Lyophilized Standard: 2
  • 100X SERPINA5 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma
• Sensitivity: 1.25 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SERPINA5
• Gene Full Name: serpin family A member 5
• Alias Symbols: acrosomal serine protease inhibitor;PAI3;PAI-3;PCI;PCI-B;PLANH3;plasma serine protease inhibitor;Plasminogen activator inhibitor 3;plasminogen activator inhibitor III;plasminogen activator inhibitor-3;PROCI;protein C inhibitor;serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5;Serpin A5;serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5.
• NCBI Gene Id: 5104
• Protein Name: Plasma serine protease inhibitor
• Description of Target: Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and proinflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary-type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue-and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as a hydrophobic hormone carrier by its binding to retinoic acid.
• Uniprot ID: P05154
• Protein Accession #: NP_000615
• Nucleotide Accession #: NM_000624