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Datasheets/Manuals | Printable datasheet for SERPINA4 Antibody (OABB00591) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Application | Immunohistochemistry|Western blot |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: Serpina 4, also called Kallistatin, PI4 or TISSUE KALLIKREIN INHIBITOR, is a protein that in humans is encoded by the SERPINA4 gene. The serpina4 is a member of the serpin superfamily and represents a major inhibitor of human tissue kallikrein in the circulation. The SERPINA4 gene is localized to 14q32.13. The translated amino acid sequence of serpina4 matches the protein sequence and shares 44 to 46% sequence identity with other serpin family members. Kallistatin is a serine proteinase inhibitor(serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. And serpina4 also plays a role in neointima hyperplasia. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Polypeptide |
Purification | Affinity Purified |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat |
Reference | 1. Chai, K. X., Chen, L.-M., Chao, J., Chao, L. Kallistatin: a novel human serine proteinase inhibitor: molecular cloning, tissue distribution, and expression in Escherichia coli. J. Biol. Chem. 268: 24498-24505, 1993. 2. Chai, K. X., Ward, D. C., Chao, J., Chao, L. Molecular cloning, sequence analysis, and chromosomal localization of the human protease inhibitor 4 (kallistatin) gene (PI4). Genomics 23: 370-378, 1994 |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Kallistatin(SERPINA4) detection. Tested with WB, IHC-P in Human. |
Gene Symbol | SERPINA4 |
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Gene Full Name | serpin family A member 4 |
Alias Symbols | KAL;kallikrein inhibitor;kallistatin;KLST;KST;peptidase inhibitor 4;PI4;PI-4;protease inhibitor 4 (kallistatin);serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4;serpin A4;serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4;sserpin family A member 4;testicular secretory protein Li 22. |
NCBI Gene Id | 5267 |
Protein Name | Kallistatin |
Description of Target | Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. |
Uniprot ID | P29622 |
Protein Accession # | NP_001275961.1; NP_001275962.1; NP_006206.2 |
Nucleotide Accession # | NM_001289032.1; NM_001289033.1; NM_006215.3 |
Molecular Weight | 48542 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SERPINA4 Antibody (OABB00591)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "SERPINA4 Antibody (OABB00591)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "SERPINA4 Antibody (OABB00591)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SERPINA4 Antibody (OABB00591)"?
This target may also be called "KAL;kallikrein inhibitor;kallistatin;KLST;KST;peptidase inhibitor 4;PI4;PI-4;protease inhibitor 4 (kallistatin);serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4;serpin A4;serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4;sserpin family A member 4;testicular secretory protein Li 22." in publications.
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What is the shipping cost for "SERPINA4 Antibody (OABB00591)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SERPINA4 Antibody (OABB00591)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SERPINA4 Antibody (OABB00591)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "48542 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SERPINA4 Antibody (OABB00591)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SERPINA4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SERPINA4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SERPINA4"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SERPINA4"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SERPINA4"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SERPINA4"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.