SERPINA1 ELISA Kit (Rat) (OKCD07605)

Catalog No: OKCD07605

Size:96WELLS

SERPINA1 ELISA Kit (Rat) (OKCD07605) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antitrypsin (a1AT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Antitrypsin (a1AT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antitrypsin (a1AT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alpha-1-Antitrypsin (a1AT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SERPINA1 Microplate: 96 Wells (12 x 8 Well strips)
  • SERPINA1 Lyophilized Standard: 2
  • 100X SERPINA1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 3.5ng/mL

Target Info

• Gene Symbol: Serpina1
• Gene Full Name: serpin family A member 1
• Alias Symbols: AAT;alpha-1-antiproteinase;Alpha-1-antitrypsin;alpha-1-antitrypsin (protease inhibitor);alpha-1-protease inhibitor;alpha-1-proteinase inhibitor;Pi;serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1;serine (or cysteine) proteinase inhibitor, clade A, member 1;serine protease inhibitor alpha 1;serpin A1;serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1;Spi1.
• NCBI Gene Id: 24648
• Protein Name: Alpha-1-antiproteinase
• Description of Target: Inhibitor of serine proteases. The primary target is elastase, but also has a moderate affinity for plasmin and thrombin.
• Uniprot ID: P17475
• Protein Accession #: NP_071964.2
• Nucleotide Accession #: NM_022519.2