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Catalog No: OKCD02332
Size:96WELLS
Price: $675.00
SKU
OKCD02332
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Datasheets/ManualsPrintable datasheet for SEPT6 ELISA Kit (Human) (OKCD02332)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Septin 6 (SEPT6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Septin 6 (SEPT6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Septin 6 (SEPT6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Septin 6 (SEPT6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Septin 6 (SEPT6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Septin 6 (SEPT6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SEPT6 Microplate96 Wells (12 x 8 Well strips)
SEPT6 Lyophilized Standard2
100X Biotinylated SEPT6 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.061 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Septin 6 (SEPT6). No significant cross-reactivity or interference between Septin 6 (SEPT6) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSEPTIN6
Gene Full Nameseptin 6
Alias SymbolsSEP2;SEPT2;SEPT6;septin 2;septin-6.
NCBI Gene Id23157
Protein NameSeptin-6
Description of TargetFilament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. May play a role in HCV RNA replication. Forms a filamentous structure with SEPT12, SEPT6, SEPT2 and probably SEPT4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830).
Uniprot IDQ14141
Protein Accession #NP_055944.2
Nucleotide Accession #NM_015129.5
Protein Size (# AA)434
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