Catalog No: OKEH01291
Size:96T
Price: $600.00
SKU
OKEH01291
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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SEPP1 ELISA Kit (Human) : 96 Wells (OKEH01291)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SEPP1 ELISA Kit (Human) (OKEH01291) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SEPP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SEPP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SEPP1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 7.1% (n = 20)
ELISA Kit Component
ComponentAmount
SEPP1 Microplate96 Wells (12 x 8 Well strips)
SEPP1 Lyophilized Standard2
100X Biotinylated SEPP1 Detector Antibody1 x 20 mL
100X Avidin-HRP Conjugate1 x 60 uL
Sample Diluent1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.089 ng/mL
SpecificityNatural and recombinant Human Selenoprotein P
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSELENOP
Gene Full Nameselenoprotein P
Alias Symbolsselenoprotein P;selenoprotein P, plasma, 1;SELP;SeP;SEPP;SEPP1.
NCBI Gene Id6414
Protein NameP49908
Description of TargetSelenoprotein P
Uniprot IDhttps://www.uniprot.org/uniprot/P49908
Protein Accession #NP_001078955.1
Nucleotide Accession #NM_001085486.1
Protein Size (# AA)381
Molecular Weight43 kDa
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