SEMA7A ELISA Kit (Human) (OKCD02881)

Catalog No: OKCD02881

Size:96WELLS

Human SEMA7A ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	83-94%	95-102%	79-93%	97-105%
  EDTA Plasma (n=5)	90-102%	78-99%	99-105%	97-105%
  Heparin Plasma (n=5)	91-98%	94-104%	78-89%	85-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 7A (SEMA7A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 7A (SEMA7A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 7A (SEMA7A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Semaphorin 7A (SEMA7A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-92	85
  EDTA Plasma (n=5)	94-105	97
  Heparin Plasma(n=5)	78-94	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 7A (SEMA7A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 7A (SEMA7A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SEMA7A Microplate: 96 Wells (12 x 8 Well strips)
  • SEMA7A Lyophilized Standard: 2
  • 100X Biotinylated SEMA7A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Semaphorin 7A (SEMA7A). No significant cross-reactivity or interference between Semaphorin 7A (SEMA7A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SEMA7A
• Gene Full Name: semaphorin 7A (John Milton Hagen blood group)
• Alias Symbols: CD108;CDw108;H-SEMA-K1;H-Sema-L;JMH;JMH blood group antigen;John Milton Hagen blood group H-Sema K1;john-Milton-Hargen human blood group Ag;sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group);sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A;sema K1;sema L;SEMAK1;SEMAL;semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group);semaphorin-7A;semaphorin-K1;semaphorin-L.
• NCBI Gene Id: 8482
• Protein Name: Semaphorin-7A
• Description of Target: Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.
• Uniprot ID: O75326
• Protein Accession #: NP_001139501.1
• Nucleotide Accession #: NM_001146029.2
• Protein Size (# AA): 666