SEMA7A Antibody (OAEE00054)

Catalog No: OAEE00054

Size:100UG

Surface staining of HPB-ALL human peripheral blood T cell leukemia cell line with anti-human CD108 (MEM-150) FITC.
Total viable cells were used for analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: MEM-150
• Isotype: IgM
• Host: Mouse
• Application: Flow cytometry|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: HPB-ALL human T cell line
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody MEM-150 reacts with CD108 (JMH blood group antigen), a 80 kDa GPI-anchored glycoprotein expressed on various cell types including erythrocytes, lymphoblasts; at low levels it is present on circulating lymphocytes.
  HLDA V; WS Code AS S017
  HLDA V; WS Code BP BP347
  HLDA VI; WS Code BP 401
  HLDA VI; WS Code BP 475
  HLDA VI; WS Code NL N-L156
  HLDA VI; WS Code P PR-65
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
• Reference: Mudad R, Rao N, Angelisova P, Horejsi V, Telen MJ.: Evidence that CDw108 membrane protein bears the JMH blood group antigen. Transfusion. 1995 Jul;35(7):566-70.
  Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  Angelisova P, Drbal K, Cerny J, Hilgert I, Horejsi V.: Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules. Immunobiology. 1999 Jun;200(2):234-45.
  Suzuki K, Okuno T, Yamamoto M, Pasterkamp RJ, Takegahara N, Takamatsu H, Kitao T, Takagi J, Rennert PD, Kolodkin AL, Kumanogoh A, Kikutani H: Semaphorin 7A initiates T-cell-mediated inflammatory responses through alpha1beta1 integrin. Nature. 2007 Apr 5;446(7136):680-4.
  Pasterkamp RJ, Kolk SM, Hellemons AJ, Kolodkin AL: Expression patterns of semaphorin7A and plexinC1 during rat neural development suggest roles in axon guidance and neuronal migration. BMC Dev Biol. 2007 Aug 29;7:98.
  Delorme G, Saltel F, Bonnelye E, Jurdic P, Machuca-Gayet I: Expression and function of semaphorin 7A in bone cells. Biol Cell. 2005 Jul;97(7):589-97.
  Pasterkamp RJ, Peschon JJ, Spriggs MK, Kolodkin AL: Semaphorin 7A promotes axon outgrowth through integrins and MAPKs. Nature. 2003 Jul 24;424(6947):398-405.

Target Info

• Gene Symbol: SEMA7A
• Gene Full Name: semaphorin 7A (John Milton Hagen blood group)
• Alias Symbols: CD108;CDw108;H-SEMA-K1;H-Sema-L;JMH;JMH blood group antigen;John Milton Hagen blood group H-Sema K1;john-Milton-Hargen human blood group Ag;sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group);sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A;sema K1;sema L;SEMAK1;SEMAL;semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group);semaphorin-7A;semaphorin-K1;semaphorin-L.
• NCBI Gene Id: 8482
• Protein Name: Semaphorin-7A
• Description of Target: Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.
• Uniprot ID: O75326
• Protein Accession #: NP_001139501.1; NP_001139502.1; NP_003603.1
• Nucleotide Accession #: NM_001146029.1; NM_001146030.1; NM_003612.3