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Datasheets/Manuals | Printable datasheet for anti-Sema5a antibody - OAAB01378 |
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Predicted Species Reactivity | Human, Mouse |
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Product Format | Liquid. PBS with 0.09% (W/V) sodium azide. |
Clonality | Polyclonal |
Isotype | Rabbit Ig |
Host | Rabbit |
Application | WB |
Additional Information | Function: Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis. Cellular Location: Membrane; Single-pass type I membrane protein. |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This Mouse Sema5a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1006-1035 amino acids from mouse Sema5a. |
Purification | This antibody was purified through a protein G column, followed by dialysis against PBS. |
Predicted Homology Based on Immunogen Sequence | Rat |
Concentration | 1.18 mg/ml |
Intended Use | For research use only and not for use in diagnostic or therapeutic procedures. |
Application Info | WB: 1:1000 |
Gene Symbol | Sema5a |
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Alias Symbols | sem, semF, Semaf, AI464145, 5930434A13, 9130201M22Rik |
NCBI Gene Id | 20356 |
Protein Name | Sema domain, seven thrombospondin repeats (Type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (Semaphorin) 5A, isoform CRA_a EMBL EDL08867.1 |
Description of Target | Members of the semaphorin protein family, such as SEMA5A, are involved in axonal guidance during neural development. |
Uniprot ID | Q3UPZ0 |
Protein Accession # | NP_033180.2 |
Molecular Weight | 121 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Sema5a Antibody (OAAB01378)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
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How long will it take to receive "Sema5a Antibody (OAAB01378)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "Sema5a Antibody (OAAB01378)" provided in?
This item is provided in "Liquid. PBS with 0.09% (W/V) sodium azide. ".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Sema5a Antibody (OAAB01378)"?
This target may also be called "sem, semF, Semaf, AI464145, 5930434A13, 9130201M22Rik" in publications.
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What is the shipping cost for "Sema5a Antibody (OAAB01378)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Sema5a Antibody (OAAB01378)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Sema5a Antibody (OAAB01378)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "121 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Sema5a Antibody (OAAB01378)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SEMA5A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SEMA5A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SEMA5A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SEMA5A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SEMA5A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SEMA5A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.