SEMA4B ELISA Kit (Human) (OKCD00640)

Catalog No: OKCD00640 (Formerly GWB-KBBRJ5)

Size:96WELLS

Human SEMA4B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4B (SEMA4B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4B (SEMA4B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 4B (SEMA4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Semaphorin 4B (SEMA4B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 4B (SEMA4B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 4B (SEMA4B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SEMA4B Microplate: 96 Wells (12 x 8 Well Strips)
  • SEMA4B Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated SEMA4B Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.071 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Semaphorin 4B (SEMA4B). No significant cross-reactivity or interference between Semaphorin 4B (SEMA4B) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SEMA4B
• Gene Full Name: semaphorin 4B
• Alias Symbols: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B;sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4B;SEMAC;semaphorin-4B;semaphorin-C;SemC.
• NCBI Gene Id: 10509
• Protein Name: Semaphorin-4B
• Description of Target: Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
• Uniprot ID: Q9NPR2
• Protein Accession #: NP_077041
• Nucleotide Accession #: NM_024127.2
• Protein Size (# AA): 461