Catalog No: OKCD09336
Size:96WELLS
Price: $750.00
SKU
OKCD09336
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Datasheets/ManualsPrintable datasheet for SEMA3B ELISA Kit (Human) (OKCD09336)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3B (SEMA3B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 3B (SEMA3B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 3B (SEMA3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Semaphorin 3B (SEMA3B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 3B (SEMA3B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 3B (SEMA3B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
SEMA3B Microplate96 Wells (12 x 8 Well strips)
SEMA3B Lyophilized Standard2
100X SEMA3B HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.056ng/mL
Gene SymbolSEMA3B
Gene Full Namesemaphorin 3B
Alias SymbolsLUCA-1;SemA;sema A(V);sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B;SEMA5;SEMAA;semaphorin A;semaphorin-3B;semaphorin-V;semaV.
NCBI Gene Id7869
Protein NameSemaphorin-3B
Description of TargetInhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Uniprot IDQ13214
Protein Accession #NP_001005914.1
Nucleotide Accession #NM_001005914.2
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