Size:96 Wells
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SEMA3A ELISA Kit (Mouse) (OKEH03495)

Catalog#: OKEH03495
Domestic: within 1 week delivery ,International: 1 week
More Information
Predicted Species ReactivityMouse
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology SEMA3A ELISA Kit (Mouse) (OKEH03495) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sema3a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Sema3a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sema3a captured in well.
Kit Range0.156 - 10ng/mL
Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Kit ReproducibilityMean Intra-assay CV%: <5.8% (n = 20)
Mean Inter-assay CV%: <8.5% (n = 24)
Kit Component
Sema3a Microplate96 Wells (12 x 8 Well strips)
Sema3a Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Sema3a Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Mouse Semaphorin-3A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSEMA3A
Official Gene Full Namesema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Alias Symbolscoll-1, collapsin-1, Hsema-I, SEMA1, Semad, Sema D, sema III, Sema III, Semaphorin-3A, Semaphorin-D, semaphorin III, Semaphorin III, SemD
NCBI Gene Id20346
Protein NameSemaphorin-3A
Description of TargetPlays a role in growth cones guidance. May function to pattern sensory projections by selectively repelling axons that normally terminate dorsally. Involved in the development of the olfactory system and in neuronal control of puberty.
Swissprot IdO08665
Protein Accession #NP_001230001.1
Nucleotide Accession #NM_001243072.1
Protein Size (# AA)772
Molecular Weight88 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Sema3a.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Sema3a.
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