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SELPLG ELISA Kit (Mouse) (OKCD00263)

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Predicted Species Reactivity:
Mouse
Application:
ELISA
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
SELPLG
Official Gene Full Name:
selectin P ligand
Alias Symbols:
CD162, P-selectin glycoprotein ligand 1, Psgl1, Psgl-1, PSGL-1, Selectin P ligand, Selp1, Selpl
NCBI Gene Id:
20345
Protein Name:
P-selectin glycoprotein ligand 1
Description of Target:
A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E- and P-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.3 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.3"P-Selectin glycoprotein ligand 1 (PSGL-1) is a physiological ligand for E-selectin in mediating T helper 1 lymphocyte migration."_x005F_x000D_ Hirata T., Merrill-Skoloff G., Aab M., Yang J., Furie B.C., Furie B._x005F_x000D_ J. Exp. Med. 192:1669-1676(2000) [PubMed] [Europe PMC] [Abstract]Cited for: INTERACTION WITH SELE AND SELP, FUNCTION.Ref.4"P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin."_x005F_x000D_ Hirata T., Furie B.C., Furie B._x005F_x000D_ J. Immunol. 169:4307-4313(2002) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.6"Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44."_x005F_x000D_ Hidalgo A., Peired A.J., Wild M.K., Vestweber D., Frenette P.S._x005F_x000D_ Immunity 26:477-489(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.
Swissprot Id:
Q62170
Protein Accession #:
NP_033177
Nucleotide Accession #:
NM_009151.3
Sample Type:
Serum, plasma and other biological fluids.
Sensitivity:
< 0.066 ng/mL
Kit Range:
0.156-10 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level Selplg were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Selplg were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology Selplg ELISA Kit (Mouse) (OKCD00263) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Selplg has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Selplg is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Selplgcaptured in well.
Kit Component:
ComponentAmount
Anti-Selplg Microplate96 Wells (12 x 8 Well Strips)
Selplg Lyophilized Standard2 x 10 ng
100X Biotinylated Selplg Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Linearity:
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)80-91%94-105%88-104%82-102%
EDTA plasma(n=5)92-101%88-98%93-105%89-103%
heparin plasma(n=5)80-90%85-97%79-104%80-94%
Kit Recovery:
MatrixRecovery Range (%)Average (%)
serum(n=5)83-10497
EDTA plasma(n=5)92-9996
heparin plasma(n=5)80-9184
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SELPLG.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SELPLG.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
This assay has high sensitivity and excellent specificity for detection of P-Selectin Glycoprotein Ligand 1 (PSGL1). No significant cross-reactivity or interference between P-Selectin Glycoprotein Ligand 1 (PSGL1) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
397

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