SELP ELISA Kit (Mouse) (OKCD06512)

Catalog No: OKCD06512

Size:96WELLS

SELP ELISA Kit (Mouse) (OKCD06512) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-Selectin (SELP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to P-Selectin (SELP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain P-Selectin (SELP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of P-Selectin (SELP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level P-Selectin (SELP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level P-Selectin (SELP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SELP Microplate: 96 Wells (12 x 8 Well strips)
  • SELP Lyophilized Standard: 2
  • 100X SELP HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 35pg/mL

Target Info

• Gene Symbol: Selp
• Gene Full Name: selectin, platelet
• Alias Symbols: CD62;CD62 antigen-like family member P;CD62P;GMP-140;Gr;granule membrane protein 140;Grmp;LECAM3;leukocyte-endothelial cell adhesion molecule 3;PADGEM;platelet activation dependent granule-external membrane protein;P-sel;P-selectin.
• NCBI Gene Id: 20344
• Protein Name: P-selectin
• Description of Target: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.
• Uniprot ID: Q01102
• Protein Accession #: NP_035477.1
• Nucleotide Accession #: NM_011347.2