SELP ELISA Kit (Bovine) (OKEH07304)

Catalog No: OKEH07304

Size:96T

Bovine SELP ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology SELP ELISA Kit (Bovine) (OKEH07304) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SELP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SELP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SELP captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.3% (n = 20)
  Mean Inter-assay CV%: <=10.7% (n = 20)
• ELISA Kit Component:
  • SELP Microplate: 96 Wells (12 x 8 Well strips)
  • SELP Lyophilized Standard: 2
  • 100X Biotinylated SELP Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  Subcellular Location: Membrane Single-pass type I membrane protein
  Unigene: Bt.4342
  SMR: P42201
  KEGG: bta:281486
  Pfam: PF00008
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.078ng/mL
• Specificity: Natural and recombinant Bovine P-selectin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SELP
• Gene Full Name: selectin P
• Alias Symbols: CD62 antigen-like family member P;GMP-140;granule membrane protein 140;LECAM3;leukocyte-endothelial cell adhesion molecule 3;PADGEM;platelet activation dependent granule-external membrane protein;P-selectin;selectin P (granule membrane protein 140kDa, antigen CD62).
• NCBI Gene Id: 281486
• Protein Name: P42201
• Description of Target: P-selectin
• Uniprot ID: https://www.uniprot.org/uniprot/P42201
• Protein Accession #: NP_776608.1
• Nucleotide Accession #: NM_174183.2