SELP Antibody (OAEE00296)

Catalog No: OAEE00296

Size:100UG

Product Info

• Predicted Species Reactivity: Human|Non-human Primate
• Product Format: Phosphate buffered saline (PBS), pH 7.4
• Clonality: Monoclonal
• Clone: AK4
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Functional assay|Immunocytochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Human platelets
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody HI62P recognizes CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.
• Formulation: Low endotoxin
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
• Reference: Ludwig RJ, Schultz JE, Boehncke WH, Podda M, Tandi C, Krombach F, Baatz H, Kaufmann R, von Andrian UH, Zollner TM: Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules. J Invest Dermatol. 2004 Mar;122(3):830-6.
  Kowalska MA, Ratajczak J, Hoxie J, Brass LF, Gewirtz A, Poncz M, Ratajczak MZ: Megakaryocyte precursors, megakaryocytes and platelets express the HIVco-receptor CXCR4 on their surface: determination of response to stromal-derived factor-1 by megakaryocytes and platelets. Br J Haematol. 1999 Feb;104(2):220-9.
  Holme PA, Müller F, Solum NO, Brosstad F, Frøland SS, Aukrust P: Enhanced activation of platelets with abnormal release of RANTES in human immunodeficiency virus type 1 infection. FASEB J. 1998 Jan;12(1):79-89.
  Dunlop LC, Skinner MP, Bendall LJ, Favaloro EJ, Castaldi PA, Gorman JJ, Gamble JR, Vadas MA, Berndt MC: Characterization of GMP-140 (P-selectin) as a circulating plasma protein. J Exp Med. 1992 Apr 1;175(4):1147-50.
  

Target Info

• Gene Symbol: SELP
• Gene Full Name: selectin P
• Alias Symbols: CD62;CD62 antigen-like family member P;CD62P;GMP140;GMP-140;granule membrane protein 140;granule membrane protein 140kDa;granulocyte membrane protein;GRMP;LECAM3;leukocyte-endothelial cell adhesion molecule 3;PADGEM;platelet activation dependent granule-external membrane protein;platelet alpha-granule membrane protein;PSEL;P-selectin;selectin P (granule membrane protein 140kDa, antigen CD62).
• NCBI Gene Id: 6403
• Protein Name: P-selectin
• Description of Target: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.
• Uniprot ID: P16109
• Protein Accession #: NP_002996.2; XP_005245492.1; XP_005245493.1
• Nucleotide Accession #: NM_003005.3; XM_005245435.1; XM_005245436.2