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Catalog No: OKCD00651 (Formerly GWB-KBBEW8)
Size:96WELLS
Price: $725.00
SKU
OKCD00651
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Datasheets/ManualsPrintable datasheet for SELE ELISA Kit (Pig) (OKCD00651)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)96-104%83-90%84-104%94-101%
EDTA plasma(n=5)97-105%83-91%87-94%82-101%
heparin plasma(n=5)93-103%84-92%78-102%80-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E-selectin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to E-selectin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E-selectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of E-selectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)90-10295
EDTA plasma(n=5)78-9288
heparin plasma(n=5)85-9889
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E-selectin were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E-selectin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SELE Microplate96 Wells (12 x 8 Well Strips)
SELE Lyophilized Standard2 x 100 ng
100X Biotinylated SELE Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity< 0.55 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Selectin, Endothelium (SELE). No significant cross-reactivity or interference between Selectin, Endothelium (SELE) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSELE
Gene Full Nameselectin E
Alias SymbolsCD62 antigen-like family member E;ELAM-1;endothelial leukocyte adhesion molecule 1;E-selectin;LECAM2;leukocyte-endothelial cell adhesion molecule 2;selectin E (endothelial adhesion molecule 1).
NCBI Gene Id397508
Protein NameE-selectin
Description of TargetCell-surface glycoprotein having a role in immunoadhesion (PubMed:7526854). Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis (By similarity).
Uniprot IDP98110
Protein Accession #NP_999433
Nucleotide Accession #NM_214268.1
Protein Size (# AA)484
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