SECTM1A ELISA Kit (Mouse) (OKWB00265)

Catalog No: OKWB00265

Size:96T

Mouse SECTM1A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	88-101%	85-104%	91-101%	96-104%
  EDTA Plasma (n=5)	83-100%	87-97%	84-97%	85-101%
  Heparin Plasma (n=5)	89-100%	88-100%	81-96%	83-100%

• ELISA Kit Principle: Aviva Systems Biology Sectm1a ELISA Kit (Mouse) (OKWB00265) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sectm1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sectm1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sectm1a captured in the well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	94-103	100
  EDTA Plasma (n=5)	85-104	96
  Heparin Plasma (n=5)	86-103	94

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Sectm1a Microplate: 96 Wells (12 x 8 Well strips)
  • Sectm1a Lyophilized Standard: 2 x 10 ng
  • 100X Sectm1a Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.094 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SECTM1A
• Alias Symbols: BC010462, MGC7970, Secreted and transmembrane protein 1A
• Protein Name: Secreted and transmembrane protein 1A
• Uniprot ID: Q921W8