SDHA Antibody (OAGA01672)

Catalog No: OAGA01672

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
OAGA01672 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
OAGA01672 diluted at 1:1000

SDHA antibody detects SDHA protein at cytosol on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
SDHA antibody (OAGA01672) dilution: 1:500.

SDHA antibody detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHA protein stained by SDHA antibody (OAGA01672) diluted at 1:500.
Blue: Hoechst 33343 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.22mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: SDHA
• Gene Full Name: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
• Alias Symbols: FP, PGL5, SDH1, SDH2, SDHF, CMD1GG, MC2DN1, NDAXOA
• NCBI Gene Id: 6389
• Description of Target: This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P31040