Catalog No: OKEH03498
Size:96T
Price: $525.00
SKU
OKEH03498
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SDC2 ELISA Kit (Mouse) (OKEH03498) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sdc2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Sdc2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sdc2 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.3% (n = 20)
Mean Inter-assay CV%: <= 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
Sdc2 Microplate96 Wells (12 x 8 Well strips)
Sdc2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Sdc2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Mouse Syndecan-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSdc2
Gene Full Namesyndecan 2
Alias Symbols4833414L08Rik;AA960457;fibro;fibroglycan;heparan sulfate proteoglycan 1, cell surface-associated (fibroglycan);heparan sulfate proteoglycan core protein;Hsp;HSPG;Hspg1;Syn;Synd2;syndecan-2.
NCBI Gene Id15529
Protein NameP43407
Description of TargetSyndecan-2
Uniprot IDhttps://www.uniprot.org/uniprot/P43407
Protein Accession #NP_032330.1
Nucleotide Accession #NM_008304.2
Protein Size (# AA)202
Molecular Weight22 kDa
Write Your Own Review
You're reviewing:SDC2 ELISA Kit (Mouse) (OKEH03498)
Your Rating
We found other products you might like!