TAF12 Antibody - N-terminal region (P100965_P050)

Data Sheet
 
Product Number P100965_P050
Product Page www.avivasysbio.com/taf12-antibody-n-terminal-region-p100965-p050.html
Name TAF12 Antibody - N-terminal region (P100965_P050)
Protein Size (# AA) 161 amino acids
Molecular Weight 18kDa
Subunit 12
NCBI Gene Id 6883
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
Description
Alias Symbols TAF2J, TAFII20
Peptide Sequence Synthetic peptide located within the following region: MNQFGPSALINLSNFSSIKPEPASTPPQGSMANSTAVVKIPGTPGAGGRL
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Denissov,S., (2007) EMBO J. 26 (4), 944-954
Description of Target Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I.Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Protein Interactions PRKAR2B; SUMO1; UBE2I; TAF4B; KAT2B; HIST3H3; SUPT3H; TAF4; ERCC1; TAF10; ELAVL1; CPSF1; TAF3; TBP; TAF1A; TAF1C; ATF7; ATXN7; NFYC; NFYB; NFYA; TAF6; TAF8; TAF9; TAF11; TAF5; TAF15; TAF7; TAF2; TAF1; MYC;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-TAF12 (P100965_P050) antibody
Blocking Peptide For anti-TAF12 (P100965_P050) antibody is Catalog # AAP31326 (Previous Catalog # AAPP02076)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TAF12
Uniprot ID Q16514
Protein Name Transcription initiation factor TFIID subunit 12
Sample Type Confirmation

TAF12 is supported by BioGPS gene expression data to be expressed in HEK293T

Protein Accession # NP_005635
Purification Affinity Purified
Nucleotide Accession # NM_005644
Tested Species Reactivity Human
Gene Symbol TAF12
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application WB, CHIP
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 85%; Rabbit: 100%; Rat: 92%
Image 1
HCT116
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Image 2
Human 293T
WB Suggested Anti-TAF12 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Transfected 293TTAF12 is supported by BioGPS gene expression data to be expressed in HEK293T
 

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