POU5F1 Antibody - N-terminal region (P100956_T100)

Data Sheet
 
Product Number P100956_T100
Product Page www.avivasysbio.com/pou5f1-antibody-n-terminal-region-p100956-t100.html
Name POU5F1 Antibody - N-terminal region (P100956_T100)
Protein Size (# AA) 360 amino acids
Molecular Weight 39kDa
NCBI Gene Id 5460
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name POU class 5 homeobox 1
Description
Alias Symbols OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4
Peptide Sequence Synthetic peptide located within the following region: PCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYT
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Suo,G., et al., (2005) Biochem. Biophys. Res. Commun. 337 (4), 1047-1051
Description of Target POU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. It determines paracrine growth factor signaling from stem cells to the trophectoderm
Protein Interactions ETS2; CETN2; XPC; RAD23B; UBC; ITCH; FOXD3; WWP2; PSMD10; NCL; PURA; PPIA; POU5F1; YBX1; KRT19; KRT14; KRT10; KRT9; KRT2; KRT1; HNRNPK; HMGB1; HIST1H1T; GAPDH; FKBP7; EHMT2; AGR2; TUBB4A; EIF5B; TUBA1A; NR2F2; TRA2B; SRSF3; RPS25; RPS23; RPS19; RPL31; RPL
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-POU5F1 (P100956_T100) antibody
Blocking Peptide For anti-POU5F1 (P100956_T100) antibody is Catalog # AAP31313 (Previous Catalog # AAPP02063)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human POU5F1
Uniprot ID Q01860
Protein Name POU domain, class 5, transcription factor 1
Publications

Kaeffer, B. et al. Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells. PLoS One 6, e25562 (2011). 22028779

Li, Y. et al. BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a dNp63+ cytotrophoblast stem cell state. Development 140, 3965-76 (2013). 24004950

Oct-4B isoform is differentially expressed in breast cancer cells: hypermethylation of regulatory elements of Oct-4A suggests an alternative promoter and transcriptional start site for Oct-4B transcription. Biosci Rep. 31, 109-15 (2011). 20433421

Protein Accession # NP_002692
Purification Protein A purified
Nucleotide Accession # NM_002701
Tested Species Reactivity Human
Gene Symbol POU5F1
Predicted Species Reactivity Human, Dog, Goat, Pig, Rabbit
Application WB
Predicted Homology Based on Immunogen Sequence Dog: 91%; Goat: 100%; Human: 100%; Pig: 84%; Rabbit: 92%
Image 1
Human Jurkat
WB Suggested Anti-POU5F1 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate
 

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