| Product Number |
P100940_P050 |
| Product Page |
www.avivasysbio.com/nfkbia-antibody-n-terminal-region-p100940-p050.html |
| Name |
NFKBIA Antibody - N-terminal region (P100940_P050) |
| Protein Size (# AA) |
317 amino acids |
| Molecular Weight |
36kDa |
| NCBI Gene Id |
4792 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| Description |
|
| Alias Symbols |
IKBA, MAD-3, NFKBI, EDAID2 |
| Peptide Sequence |
Synthetic peptide located within the following region: MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQ |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Janssen,R., et al., (2004) J. Exp. Med. 200 (5), 559-568 |
| Description of Target |
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). |
| Protein Interactions |
BTRC; NEDD9; IKBKB; CHUK; UBC; MTOR; IKBKG; SKP1; FBXW11; UBE2D3; RELA; NPLOC4; VCP; UFD1L; NFKB1; TNF; SUMO1; UBE2D1; COPS3; GPS1; SUMO3; Csnk2b; PRKCA; ST7; STAT1; REL; POLR2C; CD7; ARRB2; ARRB1; SUMO2; PSMA1; ZNF212; IKZF4; ATF4; TBK1; IKBKE; DNAJA3; S |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-NFKBIA (P100940_P050) antibody |
| Blocking Peptide |
For anti-NFKBIA (P100940_P050) antibody is Catalog # AAP31294 (Previous Catalog # AAPP02043) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human NFKBIA |
| Uniprot ID |
P25963 |
| Protein Name |
NF-kappa-B inhibitor alpha |
| Protein Accession # |
NP_065390 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_020529 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
NFKBIA |
| Predicted Species Reactivity |
Human, Pig |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Human: 100%; Pig: 76% |
| Image 1 | Human Lung
 | WB Suggested Anti-NFKBIA Antibody Titration: 0.2-1 ug/ml
Positive Control: Human Lung |
|
| Image 2 | Human Brain
 | Rabbit Anti-NFKBIA antibody
Catalog Number: P100940_P050
Paraffin Embedded Tissue: Human Brain cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 3 | Human heart
 | Rabbit Anti-NFKBIA Antibody
Catalog Number: P100940_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
