JUN Antibody - N-terminal region (P100911_P050)

• Product Number: P100911_P050
• Name: JUN Antibody - N-terminal region (P100911_P050)
• Protein Size (# AA): 331 amino acids
• Molecular Weight: 36kDa
• NCBI Gene Id: 3725
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Jun proto-oncogene
• Alias Symbols: AP1, p39, AP-1, cJUN, c-Jun
• Peptide Sequence: Synthetic peptide located within the following region: TAKMETTFYDDALNASFLPSESGPYGYSNPKILKQSMTLNLADPVGSLKP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Adiseshaiah,P., (2008) Biochem. Biophys. Res. Commun. 371 (2), 304-308
• Description of Target: JUN gene is the putative transforming gene of avian sarcoma virus 17. I JUN is highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. JUN gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: PPP3CA; SP1; HDAC1; GSK3B; FOS; ABL1; MAPK8; RFWD2; CORO7; EP300; STRN4; MAPRE3; CREB3; PRRC2A; MDM2; SMAD2; HSP90AA1; HSPA8; ATF2; APP; APLP2; GOPC; BBS7; ETS1; CREBBP; CASP9; UBC; NCOR1; EPAS1; DDIT3; BATF2; BATF3; BATF; FOSL1; SMARCD3; CEBPG; CEBPE; AT
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-JUN (P100911_P050) antibody is Catalog # AAP31260 (Previous Catalog # AAPP02009)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human JUN
• Uniprot ID: P05412
• Protein Name: Transcription factor AP-1
• Publications:

  Russo, R. et al. The newly characterized Pl-jun is specifically expressed in skeletogenic cells of the Paracentrotus lividus sea urchin embryo. FEBS J. 281, 3828-43 (2014). 25040505

• Sample Type Confirmation:

  JUN is strongly supported by BioGPS gene expression data to be expressed in HEK293T

• Protein Accession #: NP_002219
• Purification: Affinity Purified
• Nucleotide Accession #: NM_002228
• Tested Species Reactivity: Human
• Gene Symbol: JUN
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Sheep
• Application: CHIP, IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 92%; Human: 100%; Mouse: 92%; Pig: 92%; Rabbit: 92%; Rat: 92%; Sheep: 92%
• Image 1: Human Lung
  
  Rabbit Anti-JUN Antibody
  Catalog Number: P100911
  Paraffin Embedded Tissue: Human alveolar cell
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 2: Human HEK293T
  
  WB Suggested Anti-JUN Antibody Titration: 0.2-1 ug/ml
  Positive Control: Transfected 293TJUN is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells
• Image 3: HCT116
  
  Chromatin Immunoprecipitation (ChIP) Using JUN antibody - N-terminal region (P100911_P050) and HCT116 Cells