E2F1 Antibody - N-terminal region (P100821_P050)

• Product Number: P100821_P050
• Name: E2F1 Antibody - N-terminal region (P100821_P050)
• Protein Size (# AA): 437 amino acids
• Molecular Weight: 47kDa
• NCBI Gene Id: 1869
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: E2F transcription factor 1
• Description:
  View product citations for E2F1 Antibody - N-terminal region (P100821_P050) on CiteAb
• Alias Symbols: RBP3, E2F-1, RBAP1, RBBP3
• Peptide Sequence: Synthetic peptide located within the following region: PARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Hu,Y., (2008) Biochem. Biophys. Res. Commun. 370 (3), 440-444
• Description of Target: The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: RB1; SIRT1; KDM2A; LAMTOR5; EP300; KAT2B; VHL; RARA; STAT1; NCOR2; STOML1; RBL1; MAGEA11; SKP2; UHRF2; UBC; GTF2H1; ERCC3; SPT15; TFB1; TBP; NCOA3; CEBPE; PARP1; PRMT2; RBL2; CCNE1; TFDP2; TFDP1; CDKN2A; CEBPD; CDK2; CDK1; NEDD8; MCPH1; DDB2; DDB1; XIAP;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-E2F1 (P100821_P050) antibody is Catalog # AAP31170 (Previous Catalog # AAPP01913)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human E2F1
• Uniprot ID: Q01094
• Protein Name: Transcription factor E2F1
• Publications:

  Differential regulation of mammalian and avian ATOH1 by E2F1 and its implication for hair cell regeneration in the inner ear. Sci Rep. 11, 19368 (2021). 34588543

• Sample Type Confirmation:

  E2F1 is strongly supported by BioGPS gene expression data to be expressed in HEK293T

• Protein Accession #: NP_005216
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005225
• Tested Species Reactivity: Human
• Gene Symbol: E2F1
• Predicted Species Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human HEK293T
  
  WB Suggested Anti-E2F1 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:1562500
  Positive Control: Transfected 293TE2F1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells