CTNNB1 Antibody - C-terminal region (P100601_P050)

• Product Number: P100601_P050
• Name: CTNNB1 Antibody - C-terminal region (P100601_P050)
• Protein Size (# AA): 781 amino acids
• Molecular Weight: 86 kDa
• NCBI Gene Id: 1499
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Catenin (cadherin-associated protein), beta 1, 88kDa
• Description:
  View product citations for CTNNB1 Antibody - C-terminal region (P100601_P050)on CiteAb
• Alias Symbols: EVR7, CTNNB, MRD19, NEDSDV, armadillo
• Peptide Sequence: Synthetic peptide located within the following region: RTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Jiang,X., (2008) Cancer Cell 13 (6), 529-541
• Description of Target: Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-54 DA216720.1 1-54 55-2626 X87838.1 1-2572 2627-3720 AC104307.2 83770-84863
• Protein Interactions: PPARD; PLA2G4A; FBXO45; BTRC; SUMO1; UBC; HUWE1; CDH5; CDH2; TCF4; TBL1X; SOX1; SKP1; RNF220; CACYBP; FBXW11; SUMO2; SIAH1; Apc2; COPS5; RNF14; CDH1; SP1; KCTD1; MDM2; UBE2B; GSK3B; NEK2; RBX1; PTGS2; STRN3; LATS2; APC; ELAVL1; AMOT; ABL1; AXIN1; CBL; LEF
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-CTNNB1 (P100601_P050) antibody is Catalog # AAP30904 (Previous Catalog # AAPP01628)
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human CTNNB1
• Uniprot ID: P35222
• Protein Name: Catenin beta-1
• Publications:

  Repair of massively defected hemi-joints using demineralized osteoarticular allografts with protected cartilage. J Mater Sci Mater Med. 26, 227 (2015). 26319778

• Sample Type Confirmation:

  CTNNB1 is supported by BioGPS gene expression data to be expressed in HCT116

• Protein Accession #: NP_001895
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001904
• Tested Species Reactivity: Human
• Gene Symbol: CTNNB1
• Predicted Species Reactivity: Human, Mouse, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: CHIP, WB
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Zebrafish: 100%
• Image 1: Human Fetal heart
  
  WB Suggested Anti-CTNNB1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Fetal Heart
• Image 2: Human HCT116
  
  CTNNB1 antibody - middle region (P100601_P050) validated by CHIP using HCT116 cell lysate
• Image 3: Human HCT116
  
  CTNNB1 antibody - middle region (P100601_P050) validated by CHIP using HCT116 cell lysate
• Image 4: Western Blot
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.