| Product Number |
OPSN00004 |
| Product Page |
www.avivasysbio.com/sars-cov-2-spike-rbd-n501y-uk-variant-alpha-recombinant-protein-opsn00004.html |
| Name |
SARS-CoV-2 Spike RBDÃÆÃâÃâ ââ¬â¢ÃÆââââ¬Å¡Ã¬Ãâ¦Ã¡ÃÆÃââââ¬Ã
¡ÃÆââ¬Å¡ÃâàN501Y (UK Variant Alpha) Recombinant Protein (OPSN00004) |
| Protein Size (# AA) |
AA 319-537, N501Y (UK Variant Alpha) amino acids |
| Molecular Weight |
30kDa |
| Tag |
C-Avitag/His |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
43740568 |
| Host |
Sf9 Baculovirus |
| Purity |
90% |
| Concentration |
0.32 mg/mL |
| Gene Full Name |
surface glycoprotein |
| Description |
The SARS-CoV-2 Spike Receptor Binding Domain N501Y (UK Variant Alpha) Recombinant Protein (OPSN00004) is expressed in Sf9 Baculovirus cells and includes a C-terminal Avidin-His Tag with purification via IMAC. The sequence includes amino acids 319-357 of the lineage B.1.1.7 VUI-202012/01 SARS-CoV-2 spike sequence first identified in the United Kingdom. Suitable for Biotinylation and Protein:Protein Interaction assays in a variety of formats. |
| Alias Symbols |
spike glycoprotein |
| Product Format |
Liquid. 50 mM Tris-HCL, 1M NaCl, pH 7.5 |
| Reference |
A. Rambaut et al., Preliminary genomic characterisation of an emergent SARS-CoV-2 lineage in the UK defined by a novel set of spike mutations (2020), (available at https://virological.org/t/preliminary-genomic-characterisation-of-an-emergent-sars-cov-2-lineage-in-the-uk-defined-by-a-novel-set-of-spike-mutations/563). Sci Rep. 2021 Apr 28;11(1):9166. doi: 10.1038/s41598-021-88696-5. |
| Description of Target |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests. |
| Reconstitution and Storage |
Store at -20C |
| Datasheets/Manuals |
Printable datasheet for OPSN00004 |
| Additional Information |
Lineage: B.1.1.7 VUI-202012/01 |
| Formulation |
50 mM Tris-HCL, 1M NaCl, pH 7.5 |
| Uniprot ID |
P0DTC2 |
| Protein Name |
surface glycoprotein |
| Purification |
IMAC |
| Gene Symbol |
S (S1) |
| Predicted Species Reactivity |
SARS-CoV-2 |
| Application |
IA, AA, FN |
| Image 1 | S (S1) SDS-PAGE
 | Purified protein (1 ug) was loaded onto a 4-12% Bis-tris SDS-PAGE gel, electrophoresed and coomassie stained to demonstrate recombinant protein integrity and purity. Gels were imaged with a white background post destain. |
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