SARS-CoV-2 Spike RBD Recombinant Protein (OPSN00002)

Data Sheet
 
Product Number OPSN00002
Product Page www.avivasysbio.com/sars-cov-2-spike-rbd-recombinant-protein-opsn00002.html
Name SARS-CoV-2 Spike RBD Recombinant Protein (OPSN00002)
Protein Size (# AA) AA 319-537 amino acids
Molecular Weight 30kDa
Tag C-Avitag/His
Conjugation Unconjugated
NCBI Gene Id 43740568
Host Sf9 Baculovirus
Purity 90%
Concentration 0.75 mg/mL
Gene Full Name surface glycoprotein
Description The SARS-CoV-2 Spike Receptor Binding Domain Recombinant Protein (OPSN00002) is expressed in Sf9 Baculovirus cells and includes a C-terminal Avidin-His Tag with purification via IMAC. The sequence includes amino acids 319-357 of the original Wuhan SARS-CoV-2 spike sequence prior to mutation. Suitable for Biotinylation and Protein:Protein Interaction assays in a variety of formats.
Alias Symbols spike glycoprotein
Product Format Liquid. 50 mM Tris-HCL, 1M NaCl, pH 7.5
Reference SARS-CoV-2: an Emerging Coronavirus that Causes a Global Threat. Int J Biol Sci. 2020; 16(10): 1678–1685. doi: 10.7150/ijbs.45053
Description of Target Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
Reconstitution and Storage Store at -20C
Datasheets/Manuals Printable datasheet for OPSN00002
Additional Information Emergent Wuhan virus sequence
Formulation 50 mM Tris-HCL, 1M NaCl, pH 7.5
Uniprot ID P0DTC2
Protein Name surface glycoprotein
Purification IMAC
Gene Symbol S (S1)
Predicted Species Reactivity SARS-CoV-2
Application IA, AA, FN
Image 1
S (S1) SDS-PAGE
Purified protein (1 ug) was loaded onto a 4-12% Bis-tris SDS-PAGE gel, electrophoresed and coomassie stained to demonstrate recombinant protein integrity and purity. Gels were imaged with a white background post destain.
 

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