| Product Number |
OPPA01918 |
| Product Page |
www.avivasysbio.com/il-21-protein-oppa01918.html |
| Name |
IL 21 Protein (OPPA01918) |
| Protein Size (# AA) |
Recombinant amino acids |
| NCBI Gene Id |
60505 |
| Host |
E. Coli |
| Purity |
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Alias Symbols |
IL-21 |
| Peptide Sequence |
The sequence of the first five N - terminal amino acids was determined and was found to be Met - His - Lys - Ser - Ser. |
| Product Format |
Mouse IL-21 was lyophilized from 20mM NaHCO3, pH 8.5. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
| Description of Target |
Recombinant Mouse Interleukin-21 |
| Reconstitution and Storage |
Lyophilized Mouse IL-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Mouse IL21 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OPPA01918 |
| Additional Information |
Solubility: It is recommended to reconstitute the lyophilized Mouse IL21 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
| Additional Information |
Biological Activity: The ED50=10 -50 ng/ml, determined by the cell proliferation assay of modified Ba/F3 cells. |
| Additional Information |
Product Introduction: IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in na*ve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (degree C) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
Product Description: Interleukin-21 Mouse Recombinant produced in E.Coli is a single, non-glycosilated polypeptide chain containing 130 amino acids and having a total molecular mass of 15kDa. The Murine IL-21 is purified by proprietary chromatographic techniques. |
| Uniprot ID |
Q9ES17 |
| Protein Name |
Interleukin-21 |
| Protein Accession # |
NP_068554.1 |
| Gene Symbol |
IL21 |
| Predicted Species Reactivity |
Mouse |
| Image 1 | IL 21 Protein (OPPA01918)
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