| Product Number |
OPPA01717 |
| Product Page |
www.avivasysbio.com/bcl-2-protein-oppa01717.html |
| Name |
Bcl 2 Protein (OPPA01717) |
| Protein Size (# AA) |
Recombinant amino acids |
| NCBI Gene Id |
596 |
| Host |
E. Coli |
| Purity |
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Alias Symbols |
Bcl-2, PPP1R50 |
| Product Format |
The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
| Description of Target |
Recombinant Human B-Cell Leukemia/Lymphoma 2 -NWGR |
| Reconstitution and Storage |
Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Bcl-2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OPPA01717 |
| Additional Information |
Solubility: Suspend Bcl-2 in 100ul of 0.5M Acetic acid, over night at 4C. Dilute 10 fold into selected buffer system.BCL-2 has tendency to form intrumolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended. |
| Application Info |
Input marker or positive control (Western Blotting).Function study. |
| Additional Information |
Product Introduction: BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Product Description: Bcl-2 Des NWGR domain (143-146 residues) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 214 amino acids 1-142 and 147-218.The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques. |
| Uniprot ID |
P10415 |
| Protein Name |
Apoptosis regulator Bcl-2 |
| Protein Accession # |
NP_000624.2 |
| Gene Symbol |
Bcl-2 -NWGR |
| Predicted Species Reactivity |
Human |
| Application |
WB, Function Study |
| Image 1 | Bcl 2 Protein (OPPA01717)
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