| Product Number |
OPPA00647 |
| Product Page |
www.avivasysbio.com/il-4-protein-oppa00647.html |
| Name |
IL 4 Protein (OPPA00647) |
| Protein Size (# AA) |
Recombinant amino acids |
| NCBI Gene Id |
287287 |
| Host |
E. Coli |
| Purity |
Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Alias Symbols |
Il4e12 |
| Peptide Sequence |
The sequence of the first five N - terminal amino acids was determined and was found to be Met - His - Gly - Cys - Asn. |
| Product Format |
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
| Description of Target |
Recombinant Rat Interleukin-4 |
| Reconstitution and Storage |
Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL4 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OPPA00647 |
| Additional Information |
Solubility: It is recommended to reconstitute the lyophilized Interleukin 4 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
| Additional Information |
Biological Activity: The ED50 as determined by the dose-dependant prolifiration of Con A activated rat spleen cells is < 1.5 ng/ml, corresponding to a Specific Activity of 6.6 x 105 IU/mg. |
| Additional Information |
Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbencyvalue of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.
Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbencyvalue of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard. |
| Additional Information |
Product Introduction: IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Product Description: Interleukin-4 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 126 amino acids and having a molecular mass of 14105 Dalton. The IL-4 is purified by proprietary chromatographic techniques. |
| Uniprot ID |
P20096 |
| Protein Name |
Interleukin-4 |
| Protein Accession # |
NP_958427.1 |
| Gene Symbol |
IL4 |
| Predicted Species Reactivity |
Rat |
| Image 1 | IL 4 Protein (OPPA00647)
 | |
|
|
