Leptin qA Ovine Protein (OPPA00620)

• Product Number: OPPA00620
• Name: Leptin qA Ovine Protein (OPPA00620)
• Protein Size (# AA): Recombinant amino acids
• Host: E. Coli
• Purity: Greater than 98.0% as determined by (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
• Alias Symbols: Leptin qA Ovine, Leptin Antagonist Quadruple Mutant Ovine Recombinant
• Peptide Sequence: The sequence of the first five N - terminal amino acids was determined and was found to be Ala - Val - Pro - Ile - Arg.
• Product Format: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Physical appearance: White lyophilized (freeze-dried) powder.
• Description of Target: Recombinant Ovine Leptin Quadruple Antagonist
• Reconstitution and Storage: Lyophilized Leptin-Antagonist Quadruple Mutant Ovine Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0. 1 LEP-qA mutant mg/ml and up to 2 mM and filter sterilization Leptin mutant can be stored at 4C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0. 1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Leptin-Antagonist Quadruple Mutant Ovine Recombinant in sterile 0.4% NaHCO3adjusted to pH 8-9, not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Additional Information: Biological Activity: leptin qA is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of ovine leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
• Additional Information: Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
  Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Additional Information: Product Description: Leptin Antagonist Quadruple Mutant Ovine Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A/I42A mutant.Leptin Antagonist Quadruple Mutant Ovine Recombinant was purified by proprietary chromatographic techniques.
• Gene Symbol: oLeptin qA
• Predicted Species Reactivity: Sheep
• Image 1: Leptin qA Ovine Protein (OPPA00620)