Recombinant Zika Virus Envelope Protein (OPMA04848)

Data Sheet
 
Product Number OPMA04848
Product Page www.avivasysbio.com/recombinant-zika-virus-envelope-protein-opma04848.html
Name Recombinant Zika Virus Envelope Protein (OPMA04848)
Protein Size (# AA) Recombinant amino acids
Molecular Weight ~50 kDa (12.5% SDS-PAGE)
Tag Histidine tag
Purity > 80% by SDS-PAGE
Concentration Varies by lot. See vial for concentration.
Source Insect cells
Description
Peptide Sequence The amino acid sequence is proprietary
Product Format Liquid. PBS (pH 7.4) with 0.09% Sodium Azide
Description of Target The E protein composes the majority of the virion surface and it is involved with replication, such as host cell binding and membrane fusion. Covers the ectodomain of the envelope proteins. The sequence of the envelope protein is derived from the African strain (all strains share very high identity in this region).
Reconstitution and Storage Centrifuge before opening to ensure complete recovery of vial contents.
Store at -20°C, avoid repeated freeze/thaw cycles.
Thaw at room temperature or 4°C. Cryo-concentration may occur on thawing which should not affect performance characteristics. If cryo-concentration is seen after the material is completely thawed, mix by inverting the container GENTLY several times before use.
Datasheets/Manuals Printable datasheet for OPMA04848
Specificity Zika virus. Reacts with both IgM and IgG.
Additional Information Ships with Dry Ice
Application Info Suitable for use in ELISA, CLIA and Lateral Flow Assays. To overcome flavivirus cross-reactivity in diagnostics, recombinant antigens to envelope and NS1 antigens are commonly used. IgG and IgM antibodies typically show a high sensitivity and specificity to these epitopes especially NS1 which is thought to contain more species-specific epitopes than the envelope proteins. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Uniprot ID Q91KX7
Protein Name Envelope protein
Purification Purified
Predicted Species Reactivity Zika Virus
Application Chemiluminescent immunoassay, Enzyme immunoassay, Lateral flow assay
Image 1
Zika-Env
SDS-PAGE of Zika-Env
 

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