Product Number |
OPED00092 |
Product Page |
www.avivasysbio.com/mmp11-recombinant-protein-human-oped00092.html |
Name |
MMP11 Recombinant Protein (Human) (OPED00092) |
Protein Size (# AA) |
Recombinant amino acids |
Molecular Weight |
19 kDa |
NCBI Gene Id |
4320 |
Host |
E. Coli |
Purity |
>95% (SDS-PAGE) |
Concentration |
1 mg/ml |
Gene Full Name |
matrix metallopeptidase 11 |
Alias Symbols |
matrix metallopeptidase 11 (stromelysin 3);Matrix metalloproteinase-11;SL-3;ST3;STMY3;stromelysin III;stromelysin-3. |
Product Format |
Liquid 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20uM ZnCl2, 0.5% Brij-35 and 30% glycerol |
Reference |
Cancer cells, adipocytes and matrix metalloproteinase 11: a vicious tumor progression cycle: E.R. Motrescu & M.C. Rio; Biol. Chem. 389, 1037 (2008), Abstract; |
Description of Target |
May play an important role in the progression of epithelial malignancies. |
Reconstitution and Storage |
Store at -80°C |
Datasheets/Manuals |
Printable datasheet for MMP11 Recombinant Protein (Human) (OPED00092) (OPED00092) |
Application Info |
Useful to study enzyme kinetics, cleave target substrates, and screen for inhibitors. |
Biological Activity |
Due to its unusual substrate preferences [A(A/Q)(N/A)~(L/Y)(T/V/M/R)(R/K), or G(G/A)E~LR5], MMP-11 cleaves MMP peptide substrates such as Prod. No. BML-P125, BML-P126, and BML-P132 extremely slowly (several hours yield very little product). Therefore, the activity of each lot of MMP-11 is verified by digestion of macromolecules. |
Uniprot ID |
P24347 |
Protein Name |
Stromelysin-3 |
Protein Accession # |
NP_003833.4 |
Purification |
Purified by multi-step chromatography. |
Nucleotide Accession # |
NM_003842.4 |
Gene Symbol |
MMP11 |
Predicted Species Reactivity |
Homo sapiens|Human |
Image 1 | Stromelysin-3 SDS-PAGE
| Coomassie-stained SDS-PAGE showing a timecourse of α1-antitrypsin (3ug) cleavage after incubation at 37°C with or without MMP-11 catalytic domain. Lanes are as follows: 1. Marker; 2. 1.5 μg α1-antitrypsin (0 hours); 3. 50 ng MMP-11, (0 hours); 4. 1 μg MMP-11, (0 hours); 5. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (0 hours); 6. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (3 hours); 7. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (8 hours); 8. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (24 hours); 9. 1.5 μg α1-antitrypsin incubated with 1 μg MMP-11 (0 hours); 10. 1.5 μg α1-antitrypsin incubated with 1 μg MMP-11 (24 hours); 11. 1.5 μg α1-antitrypsin (24 hours); 12. Marker. |
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