MMP11 Recombinant Protein (Human) (OPED00092)

Data Sheet
 
Product Number OPED00092
Product Page www.avivasysbio.com/mmp11-recombinant-protein-human-oped00092.html
Name MMP11 Recombinant Protein (Human) (OPED00092)
Protein Size (# AA) Recombinant amino acids
Molecular Weight 19 kDa
NCBI Gene Id 4320
Host E. Coli
Purity >95% (SDS-PAGE)
Concentration 1 mg/ml
Gene Full Name matrix metallopeptidase 11
Alias Symbols matrix metallopeptidase 11 (stromelysin 3);Matrix metalloproteinase-11;SL-3;ST3;STMY3;stromelysin III;stromelysin-3.
Product Format Liquid 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20uM ZnCl2, 0.5% Brij-35 and 30% glycerol
Reference Cancer cells, adipocytes and matrix metalloproteinase 11: a vicious tumor progression cycle: E.R. Motrescu & M.C. Rio; Biol. Chem. 389, 1037 (2008), Abstract;
Description of Target May play an important role in the progression of epithelial malignancies.
Reconstitution and Storage Store at -80°C
Datasheets/Manuals Printable datasheet for MMP11 Recombinant Protein (Human) (OPED00092) (OPED00092)
Application Info Useful to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
Biological Activity Due to its unusual substrate preferences [A(A/Q)(N/A)~(L/Y)(T/V/M/R)(R/K), or G(G/A)E~LR5], MMP-11 cleaves MMP peptide substrates such as Prod. No. BML-P125, BML-P126, and BML-P132 extremely slowly (several hours yield very little product). Therefore, the activity of each lot of MMP-11 is verified by digestion of macromolecules.
Uniprot ID P24347
Protein Name Stromelysin-3
Protein Accession # NP_003833.4
Purification Purified by multi-step chromatography.
Nucleotide Accession # NM_003842.4
Gene Symbol MMP11
Predicted Species Reactivity Homo sapiens|Human
Image 1
Stromelysin-3 SDS-PAGE
Coomassie-stained SDS-PAGE showing a timecourse of α1-antitrypsin (3ug) cleavage after incubation at 37°C with or without MMP-11 catalytic domain. Lanes are as follows: 1. Marker; 2. 1.5 μg α1-antitrypsin (0 hours); 3. 50 ng MMP-11, (0 hours); 4. 1 μg MMP-11, (0 hours); 5. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (0 hours); 6. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (3 hours); 7. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (8 hours); 8. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (24 hours); 9. 1.5 μg α1-antitrypsin incubated with 1 μg MMP-11 (0 hours); 10. 1.5 μg α1-antitrypsin incubated with 1 μg MMP-11 (24 hours); 11. 1.5 μg α1-antitrypsin (24 hours); 12. Marker.
 

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