Product Number |
OPCD07299 |
Product Page |
www.avivasysbio.com/tbk1-recombinant-protein-opcd07299.html |
Name |
TBK1 Recombinant Protein (OPCD07299) |
Protein Size (# AA) |
Trp9~Val310 amino acids |
Molecular Weight |
38kDa |
Tag |
N-terminal His Tag |
Conjugation |
Unconjugated |
NCBI Gene Id |
29110 |
Host |
E.coli |
Purity |
>90% |
Concentration |
200 ug/mL (prior to lyoph) |
Source |
E.coli |
Gene Full Name |
TANK binding kinase 1 |
Alias Symbols |
FTDALS4;IIAE8;NAK;NF-kappa-B-activating kinase;NF-kB-activating kinase;serine/threonine-protein kinase TBK1;T2K;TANK-binding kinase 1. |
Product Format |
Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose. |
Description of Target |
Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy (PubMed:21617041). Phosphorylates SMCR8 component of the C9orf72-SMCR8 complex, promoting autophagosome maturation (PubMed:27103069). Phosphorylates and activates AKT1 (PubMed:21464307). Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C (PubMed:21270402). Phosphorylates Borna disease virus (BDV) P protein (PubMed:16155125). |
Reconstitution and Storage |
2°C to 8°C|-80°C |
Datasheets/Manuals |
Printable datasheet for TBK1 Recombinant Protein (OPCD07299) |
Additional Information |
Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method) Residues: Trp9-Val310 Isoelectric Point: 5.9 Subcellular Location: Cytoplasm |
Uniprot ID |
Q9UHD2 |
Protein Name |
Serine/threonine-protein kinase TBK1 |
Protein Accession # |
NP_037386.1 |
Nucleotide Accession # |
NM_013254.3 |
Gene Symbol |
TBK1 |
Predicted Species Reactivity |
Homo sapiens|Human |
Application |
Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot |
Image 1 | SDS-PAGE
| TBK1 Recombinant Protein (OPCD07299) using SDS-PAGE |
|
|