| Product Number |
OPCA335983 |
| Product Page |
www.avivasysbio.com/recombinant-sars-cov-2-spike-glycoprotein-opca335983.html |
| Name |
Recombinant SARS-CoV-2 Spike Glycoprotein (OPCA335983) |
| Protein Size (# AA) |
Recombinant amino acids |
| Molecular Weight |
51.1 kDa |
| Tag |
C-terminal 6xHis-mFc-tagged |
| NCBI Gene Id |
43740568 |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Source |
Mammalian Cells |
| Gene Full Name |
surface glycoprotein |
| Protein Range |
319-541 aa |
| Alias Symbols |
E2;GU280_gp02;Peplomer protein;spike glycoprotein;surface glycoprotein. |
| Peptide Sequence |
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF |
| Product Format |
Lyophilized |
| Description of Target |
Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32221306, PubMed:32075877, PubMed:32155444). Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell (PubMed:33082294, PubMed:33082293). This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2 (PubMed:33082293). The stalk domain of S contains three hinges, giving the head unexpected orientational freedom (PubMed:32817270). Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Can be alternatively processed by host furin (PubMed:32362314). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membrane fusion within endosomes. |
| Reconstitution and Storage |
-20°C or -80°C |
| Protein Sequence |
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF |
| Datasheets/Manuals |
Printable datasheet for Recombinant SARS-CoV-2 Spike Glycoprotein (OPCA335983) (OPCA335983) |
| Additional Information |
Biological Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ug/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 106.2-131.2 ng/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 ug/ml can bind SARS-CoV-2-S1-RBD, the EC50 of SARS-CoV-2-S1-RBD protein is 8.363-12.82 ng/ml. |
| Additional Information |
Biological Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ug/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 106.2-131.2 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 ug/ml can bind SARS-CoV-2-S1-RBD, the EC50 of SARS-CoV-2-S1-RBD protein is 8.363-12.82 ng/ml. |
| Biological Activity |
1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ug/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 106.2-131.2 ng/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 ug/ml can bind SARS-CoV-2-S1-RBD, the EC50 of SARS-CoV-2-S1-RBD protein is 8.363-12.82 ng/ml. |
| Storage Buffer |
Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Uniprot ID |
P0DTC2 |
| Protein Name |
Spike glycoprotein |
| Gene Symbol |
S |
| Predicted Species Reactivity |
SARS-CoV-2 / COVID-19 Virus|Severe acute respiratory syndrome coronavirus 2 |
| Image 1 | |
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