| Product Number |
OPCA335982 |
| Product Page |
www.avivasysbio.com/recombinant-sars-cov-2-spike-glycoprotein-opca335982.html |
| Name |
Recombinant SARS-CoV-2 Spike Glycoprotein (OPCA335982) |
| Protein Size (# AA) |
Recombinant amino acids |
| Molecular Weight |
79.6 kDa |
| Tag |
N-terminal 10xHis-tagged and C-terminal Flag-tagged |
| NCBI Gene Id |
43740568 |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Source |
Mammalian Cells |
| Gene Full Name |
surface glycoprotein |
| Protein Range |
16-685 aa |
| Alias Symbols |
E2;GU280_gp02;Peplomer protein;spike glycoprotein;surface glycoprotein. |
| Peptide Sequence |
VNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRAR |
| Product Format |
Lyophilized |
| Description of Target |
Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32221306, PubMed:32075877, PubMed:32155444). Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell (PubMed:33082294, PubMed:33082293). This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2 (PubMed:33082293). The stalk domain of S contains three hinges, giving the head unexpected orientational freedom (PubMed:32817270). Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Can be alternatively processed by host furin (PubMed:32362314). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membrane fusion within endosomes. |
| Reconstitution and Storage |
-20°C or -80°C |
| Protein Sequence |
Partial Protein: VNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRAR |
| Datasheets/Manuals |
Printable datasheet for Recombinant SARS-CoV-2 Spike Glycoprotein (OPCA335982) (OPCA335982) |
| Biological Activity |
1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S at 2 ug/ml can bind human ACE2 , the EC50 of SARS-CoV-2-S protein is 56.64 - 103.6 ng/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S at 2 ug/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S protein is 36.79-48.87 ng/ml. |
| Uniprot ID |
P0DTC2 |
| Protein Name |
Spike glycoprotein |
| Purification |
Affinity purified using IMAC |
| Gene Symbol |
S |
| Predicted Species Reactivity |
SARS-CoV-2 / COVID-19 Virus|Severe acute respiratory syndrome coronavirus 2 |
| Application |
SDS-PAGE |
| Image 1 | |
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