| Product Number |
OPCA05138 |
| Product Page |
www.avivasysbio.com/algl-recombinant-protein-azotobacter-vinelandii-opca05138.html |
| Name |
ALGL Recombinant Protein (Azotobacter vinelandii) (OPCA05138) |
| Protein Size (# AA) |
Recombinant amino acids |
| Molecular Weight |
59 kDa |
| Tag |
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Source |
E.coli |
| Protein Range |
24-374 aa |
| Alias Symbols |
Poly(beta-D-mannuronate) lyase. |
| Peptide Sequence |
AEALVPPKGYYAPVDIRKGEAPACPVVPEPFTGELVFRSKYEGSDAARSTLNEEAEKAFRTKTAPITQIERGVSRMVMRYMEKGRAGDLECTLAWLDAWAEDGALLTTEYNHTGKSMRKWALGSLAGAYLRLKFSSSQPLAAYPEQARRIESWFAKVGDQVIKDWSDLPLKRINNHSYWAAWAVMAAGVATNRRPLFDWAVEQFHIAAGQVDSNGFLPNELKRRQRALAYHNYSLPPLMMVAAFALANGVDLRGDNDGALGRLAGNVLAGVEKPEPFAERAGDEDQDMEDLETDAKFSWLEPYCALYSCSPALRERKAEMGPFKNFRLGGDVTRIFDPAEKSPRSTVGKRD |
| Product Format |
Liquid or Lyophilized powder |
| Reference |
Biochemical properties and substrate specificities of a recombinantly produced Azotobacter vinelandii alginate lyase. Ertesvag H., Erlien F., Skjak-Braek G., Rehm B.H., Valla S. J. Bacteriol. 180:3779-3784(1998) |
| Description of Target |
Catalyzes the depolymerization of alginate by cleaving the beta-1,4 glycosidic bond between two adjacent sugar residues via a beta-elimination mechanism. Splits ManA-ManA and ManA-GulA bonds, but not GulA-ManA or GulA-GulA bonds. Also cleaves acetylated residues (PubMed:9683471). May serve to degrade mislocalized alginate that is trapped in the periplasmic space (By similarity). |
| Reconstitution and Storage |
-20°C or -80°C |
| Protein Sequence |
AEALVPPKGYYAPVDIRKGEAPACPVVPEPFTGELVFRSKYEGSDAARSTLNEEAEKAFRTKTAPITQIERGVSRMVMRYMEKGRAGDLECTLAWLDAWAEDGALLTTEYNHTGKSMRKWALGSLAGAYLRLKFSSSQPLAAYPEQARRIESWFAKVGDQVIKDWSDLPLKRINNHSYWAAWAVMAAGVATNRRPLFDWAVEQFHIAAGQVDSNGFLPNELKRRQRALAYHNYSLPPLMMVAAFALANGVDLRGDNDGALGRLAGNVLAGVEKPEPFAERAGDEDQDMEDLETDAKFSWLEPYCALYSCSPALRERKAEMGPFKNFRLGGDVTRIFDPAEKSPRSTVGKRD |
| Datasheets/Manuals |
Printable datasheet for ALGL Recombinant Protein (Azotobacter vinelandii) (OPCA05138) (OPCA05138) |
| Additional Information |
Species Specificity Detail: Azotobacter vinelandii |
| Formulation |
Tris-base, 50% glycerol |
| Storage Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Uniprot ID |
O52195 |
| Protein Name |
Alginate lyase |
| Protein Accession # |
WP_012699742 |
| Nucleotide Accession # |
NZ_FPKM01000036 |
| Gene Symbol |
ALGL |
| Predicted Species Reactivity |
Azotobacter vinelandii |
| Image 1 | |
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