1.0 M Tris HCl pH 7.4 (OORA00255)

• Product Number: OORA00255
• Name: 1.0 M Tris HCl pH 7.4 (OORA00255)
• Conjugation: Unconjugated
• Concentration: 1 M
• Alias Symbols: Tris solution, THAM, tris(hydroxymethyl)aminomethane
• Product Format: Liquid (sterile filtered)
• Description of Target: Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. Tris HCl solution is useful for stabilization of buffer formulations and electrophoresis of biological molecules.
• Reconstitution and Storage: Store container at room temperature (18 to 26°C). If desired, the solution may be stored at 4°C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18 to 26°C) to restore solubility of some salts. Expiration date is six (6) months from date of opening container.
• Specificity: The product was tested on trypticase soy agar for 24 hours, 48 hours, and 72 hours, and was found to be negative for bacteria.
• Application Info: Tris solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 1.0 M Tris HCl at pH 7.4. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade (WFI) water. All assays should be optimized by the user.
• Purification: This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers.