NTH E.Coli Protein (OOPA00142)

• Product Number: OOPA00142
• Name: NTH E.Coli Protein (OOPA00142)
• Protein Size (# AA): Recombinant amino acids
• Host: Escherichia Coli.
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Alias Symbols: DNA-(apurinic or apyrimidinic site) lyase, b1633, JW1625.
• Peptide Sequence: MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I
• Product Format: Sterile Filtered colorless solution.
• Reconstitution and Storage: NTH should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Formulation: The NTH solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT, 0.1mM PMSF and 40% glycerol.
• Additional Information: Product Introduction: Nth has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme cuts impaired DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5' from the damaged site. Nth is vital for the restoration of both oxidative DNA damage and spontaneous mutagenic lesions.
  Product Description: NTH E.Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 231 amino acids (1-211a.a.) and having a molecular mass of 25.7kDa. The NTH is purified by proprietary chromatographic techniques.
• Purification: Greater than 85.0% as determined by SDS-PAGE.
• Gene Symbol: NTH