| Product Number |
OKSB00006 |
| Product Page |
www.avivasysbio.com/sba-clonotyping-system-b6-c57j-hrp-kit-oksb00006.html |
| Name |
SBA Clonotyping System-B6/C57J Kit : HRP (OKSB00006) |
| Conjugation |
HRP: Horseradish Peroxidase |
| Host |
Goat |
| Clonality |
Polyclonal |
| Application Kit |
Horseradish peroxidase (HRP)- based Enzyme- Linked- Immunosorbent- Assay(ELISA) and immunoblotting
- 1 mL goat anti- mouse Ig capture antibody (2.5 mg/mL)
- 1 mL HRP- labeled goat anti- mouse Ig screening antibody
- 1 mL HRP- labeled goat anti- mouse IgM
- 1 mL HRP- labeled goat anti- mouse IgG1
- 1 mL HRP- labeled goat anti- mouse IgG2c
- 1 mL HRP- labeled goat anti- mouse IgG2b
- 1 mL HRP- labeled goat anti- mouse IgG3
- 1 mL HRP- labeled goat anti- mouse IgA
- 1 mL HRP- labeled goat anti- mouse ?
- 1 mL HRP- labeled goat anti- mouse ?
- ABTS substrate (100 mg) |
| Reconstitution and Storage |
Store at 2-8C |
| Storage |
- The purified (UNLB) antibody is supplied 100 mM borate buffered saline, pH 8.2. No preservatives oramine- containing buffer salts added. Store at 2- 8 C
- The horseradish peroxidase (HRP) conjugates are supplied in a stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C
- All system components can be stored for several months at 2- 8 C without appreciable loss of activity
For long- term storage, the purified antibody and HRP conjugates should be aliquoted undiluted andstores at - 20 C. |
| Datasheets/Manuals |
Printable datasheet for OKSB00006 |
| Application Info |
Initial screening of fusions and selection of antibody-producing hybridomas from B6/C57J mice., Identification of the heavy and light chain isotypes of antibodies, Selection and isolation of isotype switch variants |
| Dilution |
ELISA (HRP): 1:250-1:500
Immunoblotting: Since immunoblotting methods vary, you should determine the optimum
working dilution of the product that is appropriate for your specific need. |
| Characterization |
To ensure lot- to- lot consistency, each batch of product is tested by ELISA for conformance tocharacteristics of a standard reference reagent. |
| Tested Species Reactivity |
Mouse |
| Application |
ELISA |
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