MAGIC RED CATHEPSIN B KIT (OKSA11290)

Data Sheet
 
Product Number OKSA11290
Product Page www.avivasysbio.com/magic-red-cathepsin-b-kit-oksa11290.html
Name MAGIC RED CATHEPSIN B KIT (OKSA11290)
Product Format Kit
Description of Target Cathepsin Magic RedTM kits measure cathepsin B protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product. The Magic RedTM reagent contains a cathepsin B target sequence peptide (RR)2 linked to a red (Cresyl Violet) fluorescent probe. The Cathepsin Magic RedTM kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits. This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
Reconstitution and Storage 2°C to 8°C
Datasheets/Manuals Printable datasheet for MAGIC RED CATHEPSIN B KIT (OKSA11290)
Specificity CATHEPSIN B
ELISA Kit Component Magic RedTM MR-(RR)2
Hoechst 33342 Stain
Acridine orange (AO)
Predicted Species Reactivity Human
Application IF, IHC
 

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