AGP ELISA Kit (Feline) (OKIA00195)

Data Sheet
 
Product Number OKIA00195
Product Page www.avivasysbio.com/agp-elisa-kit-feline-96-wells-okia00195.html
Name AGP ELISA Kit (Feline) (OKIA00195)
Alias Symbols AGP;agp;alpha1-gycoprotein acid;
Product Format 12 of 8-well strips
Description of Target Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain (By similarity). Appears to function in modulating the activity of the immune system during the acute-phase reaction (By similarity).
Reconstitution and Storage Store at 4 degrees
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Additional Information Intended Use: The AGP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring AGP/Alpha-1-glycoprotein acid in Feline biological samples.
Additional Information Sample Volume: 5 uL
Sensitivity 0.8 ng/mL
Uniprot ID Q6KCA0
Protein Name Alpha1-glycoprotein acid
ELISA Kit Component
ComponentAmount
Anti-Feline AGP Microplate96 Wells (12 x 8 Well Strips)
Feline AGP Calibrator1
100X AGP-HRP Conjugate150 uL
5X Assay Diluent50 mL
20X Wash Solution50 mL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
ELISA Kit Reproducibility < 10%
ELISA Kit Range 1.57 - 100 ng/mL
ELISA Kit Duration 100 Minutes
ELISA Kit Principle The Aviva Systems Biology AGP ELISA Kit (Feline) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline Alpha1-glycoprotein acid in Plasma or Serum. This assay employs an antibody specific for Feline AGP coated on a 96-well plate. Standards and samples are pipetted into the wells and Alpha1-glycoprotein acid present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-AGP antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AGP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of AGP in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
ELISA Kit Detection Method Colorimetric, OD 450 nm
Predicted Species Reactivity Feline
Application ELISA
Image 1
Representative Feline AGP Standard Curve plotted with four parameter logistic regression (4PL). Concentration is plotted as ng/mL.
Representative Feline AGP Standard Curve plotted with four parameter logistic regression (4PL). Concentration is plotted as ng/mL.
 

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