| Product Number |
OKIA00139 |
| Product Page |
https://www.avivasysbio.com/crp-elisa-kit-rat-okia00139.html |
| Name |
CRP ELISA Kit (Rat) (OKIA00139) |
| NCBI Gene Id |
25419 |
| Gene Full Name |
C-reactive protein |
| Alias Symbols |
Aa1249, Ac1262, Ab1-341, Ab2-196, Ac1-114, Ac2-069, Ba2-693 |
| Product Format |
In Solution |
| Description of Target |
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. |
| Reconstitution and Storage |
Store as indicated in product manual. |
| Datasheets/Manuals |
Printable datasheet for CRP ELISA Kit (Rat) (OKIA00139) |
| Additional Information |
Intended Use: The CRP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CRP in biological samples of rats. |
| Additional Information |
Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Rat CRP antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3â,5,5â-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RAT CRP ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat CRP.
7. RAT CRP CALIBRATOR
One vial containing a lyophilized Rat CRP calibrator. |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Sensitivity |
1.266 ng/ml |
| Uniprot ID |
P48199 |
| Protein Name |
C-reactive protein |
| Sample Type |
Serum, plasma and other biological fluids |
| ELISA Kit Component |
| Component | Amount |
|---|
| Anti-Rat CRP Microplate | 96 Wells (12 x 8 Well Strips) |
| 20X Diluent Concentrate | 50 mL |
| 20X Wash Solution | 50 mL |
| 100X Enzyme-Antibody Conjugate | 150 uL |
| Chromogen-Substrate Solution | 12 mL |
| Stop Solution | 12 mL |
| Rat CRP Calibrator | 1 |
|
| ELISA Kit Range |
6.25 ng/ml - 200 ng/ml |
| ELISA Kit Duration |
25 Minutes |
| ELISA Kit Principle |
Aviva Systems Biology CRP ELISA Kit (Rat) (OKIA00139) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for C-reactive protein has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-C-reactive protein - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C-reactive protein captured in the well. |
| ELISA Kit Detection Method |
Colorimetric, OD450 nm |
| Gene Symbol |
CRP |
| Bulk Note |
Please contact customer service when ordering 10+ kits. |
| Predicted Species Reactivity |
Rat |
| Application |
ELISA |
| Predicted Homology Based on Immunogen Sequence |
Rat |
| Image 1 | Standard curve
 | Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed. |
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