IgG ELISA Kit (Mouse) (OKIA00102)

• Name: IgG ELISA Kit (Mouse) (OKIA00102)
• Alias Symbols: IGHG1, Ig gamma, Immunoglobulin G, Ig gamma-1 chain C region
• Product Format: In Solution
• Reconstitution and Storage: Store as indicated in product manual.
• Additional Information: Intended Use: The IgG test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG in biological samples of mice. The test has minimal amount of reactivity with Bovine, Horse, Human, and Rat IgG.
• Additional Information: Reagents:
  1. DILUENT CONCENTRATE (Running Buffer)
  One bottle containing 50 ml of a 5X concentrated diluent running buffer.
  2. WASH SOLUTION CONCENTRATE
  One bottle containing 50 ml of a 20X concentrated wash solution.
  3. ENZYME-ANTIBODY CONJUGATE 100X
  One vial containing 150 ul of affinity purified anti-Mouse IgG antibody conjugated with horseradish peroxidase in a stabilizing buffer.
  4. CHROMOGEN-SUBSTRATE SOLUTION
  One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
  5. STOP SOLUTION
  One vial containing 12 ml 0.3 M sulfuric acid.
  WARNING: Avoid contact with skin.
  6. ANTI-MOUSE IgG ELISA MICRO PLATE
  Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse IgG.
  7. MOUSE IgG CALIBRATOR
  One vial containing an IgG calibrator.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Sensitivity: 2.084 ng/ml
• Protein Name: IgG
• Sample Type: Serum, plasma and other biological fluids
• ELISA Kit Component:

  Component	Amount
  Anti-Mouse IgG Microplate	96 Wells (12 x 8 Well Strips)
  20X Diluent Concentrate	50 mL
  20X Wash Solution	50 mL
  100X Enzyme-Antibody Conjugate	150 uL
  Chromogen-Substrate Solution	12 mL
  Stop Solution	12 mL
  Mouse IgG Calibrator	1

• ELISA Kit Range: 9.375 ng/ml - 600 ng/ml
• ELISA Kit Duration: 100 Minutes
• ELISA Kit Principle: Aviva Systems Biology IgG ELISA Kit (Mouse) (OKIA00102) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IgG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-IgG - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgG captured in the well.
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: Monkey
• Application: ELISA
• Predicted Homology Based on Immunogen Sequence: Mouse
• Image 1: Standard curve
  
  Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.