Plasminogen ELISA Kit (Human) (OKIA00080)

• Name: Plasminogen ELISA Kit (Human) (OKIA00080)
• NCBI Gene Id: 5340
• Gene Full Name: plasminogen
• Alias Symbols: Plasminogen, PLG, Plasmin, Plasmin light chain B
• Product Format: In Solution
• Description of Target: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
• Reconstitution and Storage: Store as indicated in product manual.
• Additional Information: Intended Use: The Plasminogen test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Plasminogen in biological samples of human.
• Additional Information: Reagents:
  1. DILUENT CONCENTRATE (Running Buffer)
  One bottle containing 50 ml of a 5X concentrated diluent running buffer.
  2. WASH SOLUTION CONCENTRATE
  One bottle containing 50 ml of a 20X concentrated wash solution.
  3. ENZYME-ANTIBODY CONJUGATE 100X
  One vial containing 150 ul of affinity purified anti-Human Plasminogen antibody conjugated with horseradish peroxidase in a stabilizing buffer.
  4. CHROMOGEN-SUBSTRATE SOLUTION
  One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
  5. STOP SOLUTION
  One vial containing 12 ml 0.3 M sulfuric acid.
  WARNING: Avoid contact with skin.
  6. ANTI-HUMAN PLASMINOGEN ELISA MICRO PLATE
  Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human PMG.
  7. HUMAN PLASMINOGEN CALIBRATOR
  One vial containing a lyophilized Human Plasminogen calibrator.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Sensitivity: 2.114 ng/ml
• Uniprot ID: P00747
• Protein Name: Plasminogen
• Sample Type: Serum, plasma and other biological fluids
• ELISA Kit Component:

  Component	Amount
  Anti-Human Plasminogen Microplate	96 Wells (12 x 8 Well Strips)
  20X Diluent Concentrate	50 mL
  20X Wash Solution	50 mL
  100X Enzyme-Antibody Conjugate	150 uL
  Chromogen-Substrate Solution	12 mL
  Stop Solution	12 mL
  Human Plasminogen Calibrator	1

• ELISA Kit Range: 6.25 ng/ml - 200 ng/ml
• ELISA Kit Duration: 130 Minutes
• ELISA Kit Principle: Aviva Systems Biology Plasminogen ELISA Kit (Human) (OKIA00080) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Plasminogen has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Plasminogen - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Plasminogen captured in the well.
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• Gene Symbol: PLG
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: Human
• Application: ELISA
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Standard curve
  
  Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.