AMH ELISA Kit (Pig) (OKEH07353)

Data Sheet
 
Product Number OKEH07353
Product Page www.avivasysbio.com/amh-elisa-kit-pig-okeh07353.html
Name AMH ELISA Kit (Pig) (OKEH07353)
Alias Symbols Anti-Muellerian hormone;Muellerian-inhibiting substance.
Description of Target Muellerian-inhibiting factor
Reconstitution and Storage Store as indicated in product manual.
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Pig Muellerian-inhibiting factor
Additional Information Function: This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
Additional Information Subcellular Location: Secreted
Additional Information Unigene: Ssc.97577
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity 0.091ng/mL
Additional Information Pfam: PF04709
Uniprot ID https://www.uniprot.org/uniprot/P79295
Protein Name P79295
ELISA Kit Component
ComponentAmount
AMH Microplate96 Wells (12 x 8 Well strips)
AMH Lyophilized Standard2
100X Biotinylated AMH Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
ELISA Kit Reproducibility Mean Intra-assay CV%: <=4.3% (n = 20)
Mean Inter-assay CV%: <=12.3% (n = 20)
ELISA Kit Range 0.312-20ng/mL
ELISA Kit Principle Aviva Systems Biology AMH ELISA Kit (Pig) (OKEH07353) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AMH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AMH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AMH captured in well.
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)104-114% (n = 20)102-111% (n = 20)101-111% (n = 20)108-118% (n = 20)
EDTA plasma(n=5)111-121% (n = 20)100-109% (n = 20)94-104% (n = 20)108-118% (n = 20)
heparin plasma(n=5)89-99% (n = 20)103-113% (n = 20)98-107% (n = 20)98-107% (n = 20)
ELISA Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20)
ELISA Kit Detection Method Colorimetric, OD450 nm
Gene Symbol AMH
Bulk Note Please contact customer service when ordering 10+ kits.
Predicted Species Reactivity Porcine, Sus scrofa
Application Enzyme-linked Immunosorbent assay-Sandwich
Image 1
Pig AMH ELISA Standard Curve
Pig AMH ELISA Standard Curve
 

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